While many protocols exist, here is a partial list of some suggested

and favorite protocols for printed DNA microarrays to get started with:

 

Local Favorites to download:

• SYTO-61 staining (html or text) for visualization of printed DNA
• 9-mer hybridization for visualization of printed DNA
• POPO™-3 Staining for visualization of printed DNA
• A Concise Guide to cDNA Microarray Analysis ­ II from Tigr.org
• Synechocystis microarray protocols from R. Burnap's Lab
• Aspergillus microarray protocols from R. Prade's Lab
• RT Direct labeling1 and Direct Labeling 2 using cy-dye dUTPs
• RT Indirect Labeling and Indirect Labeling 2 using amino allyl dUTP
• RT Indirect Labeling and Indirect Labeling 2 using amino allyl dUTP (more detailed)
• For Ambion Amino Allyl cDNA Labeling Kit
• Suggested pin condtioning protocol for OMCF
• RNA isolations using bead-milling from Dr. Hoyt's Lab
• RNA isolation from 1 to 300 cells using amplification technology
• Phalanx Array Tweaks
  

External Sites

• Tigr.org
• microarrays.org
• UMich
• stress-genomics.org   (Microarray troubleshooting image gallery)
• Comprehensive Array Manual (UCLA)